Journal: Journal of Neuroinflammation

Article Title: Anti-inflammatory and immunomodulatory mechanisms of atorvastatin in a murine model of traumatic brain injury

doi: 10.1186/s12974-017-0934-2

Figure Lengend Snippet: Effect of atorvastatin treatment on neurological outcomes after TBI. a A timeline of the experimental design. i.g.: intragastric gavage. b , c Representative images of the cortical contusion region induced by a CCI system. Shaded areas illustrate the peri-contusional cortex that was harvested for ELISA and qRT-PCR analysis ( b ) and the microphotographed areas used in immunohistochemistry and immunofluorescence ( c ). d, e The neurological recovery was analyzed by mNSS ( d ) and Rota-rod( e ) tests prior to and at 24 and 72 h post-TBI. d mNSS scores were significantly higher in the four TBI groups compared with those in the sham groups. No significant differences were observed among the four TBI groups at 24 h. However, the three different doses of atorvastatin groups showed significantly lower mNSS scores compared with those in the saline group at 72 h post-TBI. No significant differences were detected among the 1, 5, and 10 mg/kg/day atorvastatin groups at any time point. e Compared with the mice in the TBI + saline group, atorvastatin (1, 5, and 10 mg/kg/day) administration attenuated the TBI-induced impaired Rota-rod performance at 24 and 72 h after TBI. However, differences in Rota-rod latency among the three atorvastatin groups were not significant on any testing day. Data are presented as the mean ± SD. *** p < 0.001 versus sham group, # p < 0.05 and ## p < 0.01 versus TBI + saline group. n = 24/group (mNSS) or 12/group (Rota-rod)

Article Snippet: *** p < 0.001 versus sham group, # p < 0.05 and ## p < 0.01 versus TBI + saline group. n = 24/group (mNSS) or 12/group (Rota-rod) To determine the efficacy of atorvastatin (Atorvastatin calcium tablet, Pfizer Inc.; dissolved in 0.9% saline) on neuroinflammation in the acute phase of TBI, the initial dose was administered orally by intragastric gavage (i.g.) as early as 1 h after TBI [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Immunohistochemistry, Immunofluorescence, Saline

Journal: Journal of Neuroinflammation

Article Title: Anti-inflammatory and immunomodulatory mechanisms of atorvastatin in a murine model of traumatic brain injury

doi: 10.1186/s12974-017-0934-2

Figure Lengend Snippet: Effect of atorvastatin treatment on brain immune cell subsets after TBI. a Representative gating strategy of isolating microglia (CD11b + CD45 low ), macrophages (CD11b + CD45 high Ly6G − ), neutrophils (CD11b + CD45 high Ly6G + ), total T cells (CD45 + CD3 + ), B cells (CD45 + B220 + ), and NK cells (CD45 + NK1.1 + ) infiltrating the brain. b – g Quantitative analysis of the invading cellular components in the different groups. Flow cytometric analysis showed a significant decline in microglia ( b ), macrophages ( c ), and T cells ( b ) in the atorvastatin group compared with the saline group at 72 h post-TBI. Atorvastatin administration had no effect on B cells ( f ) at 24 and 72 h following TBI. At 24 and 72 h post-TBI, atorvastatin treatment led to a significant reduction in the recruitment of neutrophils ( d ) and NK cells ( g ) to the injured brain when compared to the saline group. Data are presented as the mean ± SD. ** p < 0.01 and *** p < 0.001 versus sham group, # p < 0.05, ## p < 0.01, and ### p < 0.001 versus TBI + saline group. n = 6/group. FSC-A = forward scatter channel area, SSC-A = side scatter channel area, FITC = fluorescein isothiocyanate, PE = phycoerythrin, and APC = allophycocyanin

Article Snippet: *** p < 0.001 versus sham group, # p < 0.05 and ## p < 0.01 versus TBI + saline group. n = 24/group (mNSS) or 12/group (Rota-rod) To determine the efficacy of atorvastatin (Atorvastatin calcium tablet, Pfizer Inc.; dissolved in 0.9% saline) on neuroinflammation in the acute phase of TBI, the initial dose was administered orally by intragastric gavage (i.g.) as early as 1 h after TBI [ ].

Techniques: Saline

Journal: Journal of Neuroinflammation

Article Title: Anti-inflammatory and immunomodulatory mechanisms of atorvastatin in a murine model of traumatic brain injury

doi: 10.1186/s12974-017-0934-2

Figure Lengend Snippet: Effect of atorvastatin treatment on leukocyte invasion after TBI. a , b Representative immunohistochemically photomicrographs of neutrophils (MPO + , a ) and T cells (CD3 + , b ) in the peri-contusional cortex after TBI. Scale bar = 200 μm. Inset display high magnification images of a positive cell. c , d Cell count analysis of neutrophils ( c ) and T cells ( d ) in the different groups. Atorvastatin-treated mice had significantly fewer infiltrated neutrophils (at 24 h), and infiltrated T cells (at 72 h) compared with those in saline-treated mice after TBI. Data are presented as the mean ± SD. *** p < 0.001 versus sham group, # p < 0.05 versus TBI + saline group. n = 6/group

Article Snippet: *** p < 0.001 versus sham group, # p < 0.05 and ## p < 0.01 versus TBI + saline group. n = 24/group (mNSS) or 12/group (Rota-rod) To determine the efficacy of atorvastatin (Atorvastatin calcium tablet, Pfizer Inc.; dissolved in 0.9% saline) on neuroinflammation in the acute phase of TBI, the initial dose was administered orally by intragastric gavage (i.g.) as early as 1 h after TBI [ ].

Techniques: Cell Counting, Saline

Journal: Journal of Neuroinflammation

Article Title: Anti-inflammatory and immunomodulatory mechanisms of atorvastatin in a murine model of traumatic brain injury

doi: 10.1186/s12974-017-0934-2

Figure Lengend Snippet: Effect of atorvastatin treatment on Tregs in the spleen and brain after TBI. a, b Representative dot plots showing the gating strategy of CD4 + CD25 + Foxp3 + Tregs from the peripheral spleen ( a ) and brain ( b ). Data are expressed as the Tregs in CD4+ T cells (%). c, d Quantitative analysis of the Tregs in the spleen and brain in the different groups. Mice in the TBI + saline group showed a decreased percentage of CD25 + Foxp3+ Tregs in spleen CD4+ T cells at 24 and 72 h ( c ); however, an increased percentage of Tregs in CD4+ T cells were present in the brain ( d ) at 72 h post-injury compared with mice in the sham groups. Atorvastatin treatment post-TBI increased the proportions of CD25 + Foxp3+ Tregs among CD4+ T cells significantly in both the spleen ( c ) and brain (at 72 h, d ), compared with the TBI + saline group. Data are presented as the mean ± SD. * p < 0.05, ** P < 0.01, and *** P < 0.001 versus sham group, ### P < 0.001 versus TBI + saline group. n = 6/group. FSC-A = forward scatter channel area, SSC-A = side scatter channel area, FITC = fluorescein isothiocyanate, PE = phycoerythrin, and APC = allophycocyanin

Article Snippet: *** p < 0.001 versus sham group, # p < 0.05 and ## p < 0.01 versus TBI + saline group. n = 24/group (mNSS) or 12/group (Rota-rod) To determine the efficacy of atorvastatin (Atorvastatin calcium tablet, Pfizer Inc.; dissolved in 0.9% saline) on neuroinflammation in the acute phase of TBI, the initial dose was administered orally by intragastric gavage (i.g.) as early as 1 h after TBI [ ].

Techniques: Saline

Journal: Journal of Neuroinflammation

Article Title: Anti-inflammatory and immunomodulatory mechanisms of atorvastatin in a murine model of traumatic brain injury

doi: 10.1186/s12974-017-0934-2

Figure Lengend Snippet: Effect of atorvastatin treatment on inflammatory cytokines expression in peri-contusional cortex after TBI. a – d Compared with the TBI + saline group, atorvastatin treatment increased the concentrations of the anti-inflammatory cytokines TGF-β1 ( a ) and IL-10 ( b ) significantly, whereas it reduced the levels of pro-inflammatory cytokines IFN-γ ( c ) and IL-6 ( d ) in the contusional boundary at 24 and 72 h after TBI. e-f The chemokines RANTES ( e ) and IP-10 ( f ) were also reduced at 24 and 72 h after TBI in the atorvastatin group compared with the TBI + saline group. Data are presented as the mean ± SD. *** P < 0.001 versus sham group, # p < 0.05, ## p < 0.01, and ### P < 0.001 versus TBI + saline group. n = 6/group

Article Snippet: *** p < 0.001 versus sham group, # p < 0.05 and ## p < 0.01 versus TBI + saline group. n = 24/group (mNSS) or 12/group (Rota-rod) To determine the efficacy of atorvastatin (Atorvastatin calcium tablet, Pfizer Inc.; dissolved in 0.9% saline) on neuroinflammation in the acute phase of TBI, the initial dose was administered orally by intragastric gavage (i.g.) as early as 1 h after TBI [ ].

Techniques: Expressing, Saline

Journal: Journal of Neuroinflammation

Article Title: Anti-inflammatory and immunomodulatory mechanisms of atorvastatin in a murine model of traumatic brain injury

doi: 10.1186/s12974-017-0934-2

Figure Lengend Snippet: Effect of atorvastatin treatment on microglia/macrophage activation after TBI. a Representative immunohistochemical photomicrographs of Iba-1 stained microglia/macrophages in the peri-contusional cortex after TBI. Scale bar = 200 μm (upper,) and 100 μm (lower). Inset display high magnification images of a positive cell. Morphological observation showed that TBI provoked a drastic change in the morphology of microglia from the surveillant and ramified shape to a round and enlarged appearance. At 72 h after TBI, treatment with atorvastatin significantly reduced the soma size and ramification index. b Quantification of Iba-1 positive cells in the different groups. Atorvastatin-treated mice had significantly fewer activated microglia/macrophages compared with those in saline-treated mice at 72 h after TBI. Data are presented as the mean ± SD. *** p < 0.001 versus sham group, # p < 0.05 versus TBI + saline group. n = 6/group

Article Snippet: *** p < 0.001 versus sham group, # p < 0.05 and ## p < 0.01 versus TBI + saline group. n = 24/group (mNSS) or 12/group (Rota-rod) To determine the efficacy of atorvastatin (Atorvastatin calcium tablet, Pfizer Inc.; dissolved in 0.9% saline) on neuroinflammation in the acute phase of TBI, the initial dose was administered orally by intragastric gavage (i.g.) as early as 1 h after TBI [ ].

Techniques: Activation Assay, Immunohistochemical staining, Staining, Saline

Journal: Journal of Neuroinflammation

Article Title: Anti-inflammatory and immunomodulatory mechanisms of atorvastatin in a murine model of traumatic brain injury

doi: 10.1186/s12974-017-0934-2

Figure Lengend Snippet: Effects of atorvastatin treatment on M1/M2 microglia/macrophage polarization after TBI. a Representative gating strategy of M1 microglia/macrophages (CD11b + CD86 + ), and M2 microglia/macrophages (CD11b + CD206 + ). b – d Quantitative analysis of the M1, M2 cells and the ratio of M2/M1 in the different groups. Atorvastatin-treated mice had significantly fewer M1 cells, more M2 cells, and higher M2/M1 ratio compared with saline-treated mice at at 72 h after TBI. e qRT-PCR results for the M1-type mRNA expression of MCP-1, iNOS and CD11b. f M2-type mRNA expression of Arg1, Ym1/2 and CD206. Expression levels of the mRNAs were normalized to that in the sham control. TBI induced a marked increase in both M1- and M2-type mRNA expression in the injured brains of mice compared with the sham groups. However, atorvastatin administration significantly attenuated M1 related gene expressions and promoted M2 related gene expressions compared with those in the TBI + saline group at 72 h post-injury. Data are presented as the mean ± SD. ** P < 0.01 and *** p < 0.001 versus sham group, # p < 0.01, ## p < 0.01, and ### P < 0.001 versus TBI + saline group. n = 6/group

Article Snippet: *** p < 0.001 versus sham group, # p < 0.05 and ## p < 0.01 versus TBI + saline group. n = 24/group (mNSS) or 12/group (Rota-rod) To determine the efficacy of atorvastatin (Atorvastatin calcium tablet, Pfizer Inc.; dissolved in 0.9% saline) on neuroinflammation in the acute phase of TBI, the initial dose was administered orally by intragastric gavage (i.g.) as early as 1 h after TBI [ ].

Techniques: Saline, Quantitative RT-PCR, Expressing, Control

Journal: Journal of Neuroinflammation

Article Title: Anti-inflammatory and immunomodulatory mechanisms of atorvastatin in a murine model of traumatic brain injury

doi: 10.1186/s12974-017-0934-2

Figure Lengend Snippet: Effect of atorvastatin treatment on neuronal apoptosis after TBI. a Representative fluorescence images of TUNEL-positive neurons in the peri-contusional cortex at 72 h after TBI. Fluorescence colors: TUNEL: red, NeuN: green, and DAPI: blue. Scale bar = 200 μm. TUNEL and NeuN double stained cells indicated the apoptotic neurons, overlapped images showed that TUNEL-positive cells mainly colocalized with neurons. b Quantitative analysis of apoptotic neurons in the different groups. Few TUNEL-positive apoptotic neurons were detected in the sham groups. Apoptotic neurons in the peri-contusional cortex at 72 h post-TBI were reduced in the atorvastatin treatment group compared with those in the saline group. c The ratio of apoptotic neurons in total neurons. Compared with the TBI + saline group, administration of atorvastatin significantly decreased the apoptosis ratio. Data are presented as the mean ± SD. *** p < 0.001 versus sham group, ## p < 0.01 and ### P < 0.001 versus TBI + saline group. n = 6/group

Article Snippet: *** p < 0.001 versus sham group, # p < 0.05 and ## p < 0.01 versus TBI + saline group. n = 24/group (mNSS) or 12/group (Rota-rod) To determine the efficacy of atorvastatin (Atorvastatin calcium tablet, Pfizer Inc.; dissolved in 0.9% saline) on neuroinflammation in the acute phase of TBI, the initial dose was administered orally by intragastric gavage (i.g.) as early as 1 h after TBI [ ].

Techniques: Fluorescence, TUNEL Assay, Staining, Saline